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首頁 > 產(chǎn)品中心 > 免疫學 > 抗體 > A00186THE™ His Tag Antibody, mAb, Mouse

THE™ His Tag Antibody, mAb, Mouse

描述:THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.

更新時間:2024-09-10
產(chǎn)品型號:A00186
廠商性質(zhì):代理商
詳情介紹
品牌GenScript/金斯瑞貨號A00186
規(guī)格100ug供貨周期一周
應用領域生物產(chǎn)業(yè)

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THE™ 代表了"THE unmistakable, THE irreplaceable, THE best quality"。金斯瑞選擇穩(wěn)定、高產(chǎn)的雜交瘤細胞克隆,然后使用相應的抗原進行篩選分離,制備出特異性高、親和力強的THE™ 系列金牌抗體。

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主要特色

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Description

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.
           THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.
           THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.
           THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Host SpeciesMouse
ConjugationUnconjugated
ImmunogenA synthetic peptide HHHHHH coupled to KLH
Fusion PartnerSpleen cells were fused with SP2/0-Ag14 mouse myeloma cells
PurificationProtein A affinity column



SubclassIgG1, k
Clone ID6G2A9
Concentration0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
ReconstitutionReconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
SpecificityTHE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
StorageThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
NoteGenScript can offer this product according to your requirement, including product size, buffer components,etc.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
           ELISA: 0.05-0.2 µg/ml
           Western Blot: 0.1-0.2 µg/ml
           Immunoprecipitation (IP): 1 µg/ml
           Immunofluorescent staining: 1 µg/ml
           Flow cytometry (FACS): 1 µg/ml
           TR-FRET assays: user-optimized
           Other applications: user-optimized
           
           

















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